Cooperative mechanism in epigenetic network controls gene expression quantity.
- LecturerProf. Mariko Okada (Institute for Protein Research, Osaka University)
Host: Huai-Kuang Tsai - Time2019-11-20 (Wed.) 10:30 ~ 12:30
- LocationAuditorium106 at IIS new Building
Abstract
Gene expression is controlled by signaling activities and epigenetic regulation. To understand quantitative mechanism of gene regulatory machinery, we analyze multi-omics data on histone modification, chromatin accessibility and single cell mRNA expression using a mathematical model. Our study using antigen-stimulated B cell indicated that multiple binding of NF-kB transcription factor on chromatin accessible region of the longer size of enhancer can induce higher level of gene expression than that is controlled by fewer binding of the NF-kB on the shorter size of enhancer. Our analysis indicates that the enhancer length is critical for quantitative control of gene expression. Imaging analysis further indicated that a cooperativity in transcription factor interaction arises from liquid-liquid phase separation in nucleus might be responsible for the event.
BIO
1988 Graduate School, Tokyo University of Agriculture and Technology (Major in Biochemistry), Japan
1988 – 1996 Research Scientist, Group Leader, Manager, Novo Nordisk Bioindustry, Ltd.
1996 – 1999 Visiting Researcher, Institute of Toxicology and Environmental Health, University of California, Davis, USA.
2000 – 2016 Research Scientist, Senior Research Scientist, Team leader, RIKEN Institute, Japan
2016 – present Professor, Institute for Protein Research, Osaka University, Japan